Biospecimen Processing Protocol / Clinical Data

Biospecimen Processing Protocol
Clinical Data

Biospecimen Processing Protocol
Blood samples from biomarker participants are transported at room temperature to the Biomarkers Core laboratory for processing within 4 hours of blood draw. They are centrifuged and aliquotted into plasma, packed red blood cells, buffy coat and serum and stored at -80°. Samples collected in PAXgene tubes are transferred at room temperature to the laboratory and placed at -4° within 4 hours of the blood draw. RNA is processed within 3-5 days by the biospecimen manager using the PreAnalytix manual PAXgene blood RNA procedure. Cell lines are established and stored.  CSF is available from a subset of subjects.


Photographer: Sam Ogden

Clinical Data
Clinical data collected for every enrolled subject comes from physician exams measuring disease activity and severity, study staff interviews, and self-administered questionnaires and includes medical history, medication use, risk factor data, family history of neurologic diseases, co-morbidities, smoking history and caffeine intake. Also included are standardized tests measuring quality of life, depression, physical and cognitive function.